| POS. | # | RIDER | HOMETOWN | BIKE | TEAM/SPONSORS |
|---|---|---|---|---|---|
| Start Position 1 | 23 | Chase Sexton | Clermont, FL | Honda CRF450R WE | Team Honda HRC |
| Start Position 2 | 3 | Eli Tomac | Cortez, CO | Yamaha YZ450F | Monster Energy Star Racing Yamaha |
| Start Position 3 | 21 | Jason Anderson | Rio Rancho, NM | Kawasaki KX450SR | Monster Energy Kawasaki |
| Start Position 4 | 94 | Ken Roczen | Clermont, FL | Honda CRF450R WE | Team Honda HRC |
| Start Position 5 | 7 | Aaron Plessinger | Hamilton, OH | KTM 450 SX-F FE | Red Bull KTM |
| Start Position 6 | 51 | Justin Barcia | Greenville, FL | GASGAS MC 450F | Troy Lee Designs Red Bull GASGAS Factory Racing |
| Start Position 7 | 27 | Malcolm Stewart | Murrieta, CA | Husqvarna FC 450 RE | Rockstar Energy Husqvarna Factory Racing |
| Start Position 8 | 9 | Adam Cianciarulo | New Smyrna Beach, FL | Kawasaki KX450SR | Monster Energy Kawasaki |
| Start Position 9 | 14 | Dylan Ferrandis | Tallahassee, FL | Yamaha YZ450F | Monster Energy Star Racing Yamaha |
| Start Position 10 | 1 | Cooper Webb | Newport, NC | KTM 450 SX-F FE | Red Bull KTM |
| Start Position 11 | 15 | Dean Wilson | Murrieta, CA | Husqvarna FC 450 RE | Rockstar Energy Husqvarna Factory Racing |
| Start Position 12 | 25 | Marvin Musquin | Corona, CA | KTM 450 SX-F FE | Red Bull KTM |
| Start Position 13 | 19 | Justin Bogle | Wesley Chapel, FL | Suzuki RM-Z450 | Twisted Tea Suzuki |
| Start Position 14 | 12 | Shane McElrath | Oakland, FL | KTM 450 SX-F FE | Rocky Mountain ATV/MC WPS KTM |
| Start Position 15 | 11 | Kyle Chisholm | Valrico, FL | Yamaha YZ450F | Team Chiz, Yamaha, LS2, FXR, Rock River |
| Start Position 16 | 17 | Joey Savatgy | Clermont, FL | KTM 450 SX-F FE | Rocky Mountain ATV/MC WPS KTM |
| Start Position 17 | 34 | Max Anstie | Murrieta, CA | KTM 450 SX-F | Rocky Mountain ATV/MC WPS KTM |
| Start Position 18 | 41 | Brandon Hartranft | Brick, NJ | Suzuki RM-Z450 | Twisted Tea Suzuki |
| Start Position 19 | 26 | Alex Martin | Clermont, FL | Yamaha YZ450F | Muc-Off FXR ClubMX |
| Start Position 20 | 751 | Josh Hill | Huntersville, NC | KTM 450 SX-F | Team Tedder Monster Energy Lucas Oil KTM Racing |
| Start Position 21 | 95 | Justin Starling | Deland, FL | GASGAS MC 450F | FXR, SKDA, JSR Motorsports |
| Start Position 22 | 848 | Joan Cros | Manlleu, BC | Kawasaki KX450 | MADD Parts, Circuit Equipment, Namura Tech, Kawasaki |