| POS. | # | RIDER | HOMETOWN | BIKE | TEAM/SPONSORS |
|---|---|---|---|---|---|
| Start Position 1 | 33 | Austin Forkner | Richards, MO | Kawasaki KX250 | Monster Energy Pro Circuit Kawasaki |
| Start Position 2 | 28 | Christian Craig | Temecula, CA | Yamaha YZ250F | Monster Energy Star Racing Yamaha |
| Start Position 3 | 54 | Mitchell Oldenburg | Aledo, TX | Honda CRF250R | Smartop Bullfrog Spas MotoConcepts Honda |
| Start Position 4 | 49 | Nate Thrasher | Livingston, TN | Yamaha YZ250F | Monster Energy Star Racing Yamaha |
| Start Position 5 | 80 | Enzo Lopes | Indian Trail, NC | Yamaha YZ250F | Muc-Off FXR ClubMX, IAMACOMEBACK, Yamaha |
| Start Position 6 | 66 | Chris Blose | Phoenix, AZ | GASGAS MC 250F | AJE Motorsport MOTUL GAS GAS |
| Start Position 7 | 90 | Jordon Smith | Ochlocknee, GA | Honda CRF250R | Fire Power Honda |
| Start Position 8 | 30 | Jo Shimoda | Menifee, CA | Kawasaki KX250 | Monster Energy Pro Circuit Kawasaki |
| Start Position 9 | 81 | Jace Owen | Mattoon, IL | Yamaha YZ250F | Muc-Off FXR ClubMX, IAMACOMEBACK, Yamaha |
| Start Position 10 | 98 | Dominique Thury | Schneeberg, GER | Yamaha YZ250F | Muc-Off FXR ClubMX, IAMACOMEBACK, Yamaha |
| Start Position 11 | 285 | Marshal Weltin | Ubly, MI | Yamaha YZ250F | SRS |
| Start Position 12 | 910 | Carson Brown | Ravensdale, WA | KTM 250 SX-F | AEO Powersports KTM Racing |
| Start Position 13 | 331 | Derek Drake | Lake Elsinore, CA | Suzuki RM-Z250 | Bar X Suzuki |
| Start Position 14 | 96 | Hunter Lawrence | Wesley Chapel, FL | Honda CRF250R | Team Honda HRC |
| Start Position 15 | 24 | Rj Hampshire | Minneola, FL | Husqvarna FC 250 | Rockstar Energy Husqvarna Factory Racing |
| Start Position 16 | 62 | Vince Friese | Menifee, CA | Honda CRF250R | Smartop Bullfrog Spas MotoConcepts Honda |
| Start Position 17 | 39 | Jarrett Frye | Mechanicsville, MD | Honda CRF250R | Fire Power Honda |
| Start Position 18 | 31 | Jalek Swoll | Clermont, FL | Husqvarna FC 250 | Rockstar Energy Husqvarna Factory Racing |
| Start Position 19 | 44 | Pierce Brown | Sandy, UT | GASGAS MC 250F | Troy Lee Designs Red Bull GASGAS Factory Racing |
| Start Position 20 | 29 | Michael Mosiman | Minneaola, FL | GASGAS MC 250F | Troy Lee Designs Red Bull GASGAS Factory Racing |
| Start Position 21 | 140 | Cullin Park | Clermont, FL | Honda CRF250R | Phoenix Racing Honda |
| Start Position 22 | 64 | Mitchell Harrison | Leesburg, FL | GASGAS MC 250F | AJE Motorsport MOTUL GAS GAS |