| POS. | # | RIDER | HOMETOWN | BIKE | TEAM/SPONSORS |
|---|---|---|---|---|---|
| Start Position 1 | 1 | Eli Tomac | Cortez, CO | Yamaha YZ450F | Monster Energy Yamaha Star Racing |
| Start Position 2 | 27 | Malcolm Stewart | Haines City, FL | Husqvarna FC 450 RE | Rockstar Energy Husqvarna Factory Racing |
| Start Position 3 | 21 | Jason Anderson | Rio Rancho, NM | Kawasaki KX450SR | Monster Energy Kawasaki |
| Start Position 4 | 94 | Ken Roczen | Clermont, FL | Suzuki RM-Z450 | Progressive Insurance Ecstar Suzuki |
| Start Position 5 | 51 | Justin Barcia | Greenville, FL | GASGAS MC 450F | Troy Lee Designs Red Bull GASGAS Factory Racing |
| Start Position 6 | 9 | Adam Cianciarulo | New Smyrna Beach, FL | Kawasaki KX450SR | Monster Energy Kawasaki |
| Start Position 7 | 45 | Colt Nichols | Murrieta, CA | Honda CRF450R | Team Honda HRC |
| Start Position 8 | 14 | Dylan Ferrandis | Tallahassee, FL | Yamaha YZ450F | Monster Energy Yamaha Star Racing |
| Start Position 9 | 28 | Christian Craig | Clermont, FL | Husqvarna FC 450 RE | Rockstar Energy Husqvarna Factory Racing |
| Start Position 10 | 2 | Cooper Webb | Newport, NC | KTM 450 SX-F FE | Red Bull KTM |
| Start Position 11 | 7 | Aaron Plessinger | Hamilton, OH | KTM 450 SX-F FE | Red Bull KTM |
| Start Position 12 | 17 | Joey Savatgy | Clermont, FL | Kawasaki KX450SR | Rick Ware Racing |
| Start Position 13 | 519 | Joshua Cartwright | Fort Worth, TX | Kawasaki KX450 | Psychic Motorsports, T3 Civil, Ronnie Prado Company, Cardinal |
| Start Position 14 | 46 | Justin Hill | Yoncalla, OR | KTM 450 SX-F | Team Tedder Monster Energy Mountain Motorsports |
| Start Position 15 | 47 | Fredrik Noren | Indian Trail, NC | Kawasaki KX450SR | MaddParts.com, Big Buildings Direct, RPC Kawasaki |
| Start Position 16 | 15 | Dean Wilson | Menifee, CA | Honda CRF450R | Fire Power Honda |
| Start Position 17 | 140 | Alex Ray | Milan, TN | Yamaha YZ450F | Swapmoto Live, ONEAL, Yamaha |
| Start Position 18 | 11 | Kyle Chisholm | Valrico, FL | Suzuki RM-Z450 | Twisted Tea Suzuki Progressive Insurance |
| Start Position 19 | 23 | Chase Sexton | Clermont, FL | Honda CRF450R | Team Honda HRC |
| Start Position 20 | 73 | John Short | Pilot Point, TX | Kawasaki KX450SR | MaddParts.com, Big Buildings Direct, RPC Kawasaki |
| Start Position 21 | 12 | Shane McElrath | Oakland, FL | Suzuki RM-Z450 | Twisted Tea Suzuki Progressive Insurance |
| Start Position 22 | 60 | Justin Starling | Riverview, FL | GASGAS MC 450F | FXR, General Grind & Machine, JSR Motorsports |