| POS. | # | RIDER | HOMETOWN | BIKE | TEAM/SPONSORS |
|---|---|---|---|---|---|
| Start Position 1 | 42 | Joshua Varize | Perris, CA | KTM 250 SX-F | AEO Powersports |
| Start Position 2 | 66 | Henry Miller | Rochester, MN | Honda CRF250R | Ti-Lube, Storm Lake Honda, Innovation Concrete Inc, Rangeline |
| Start Position 3 | 410 | Brandon Scharer | Gentry, AR | Suzuki RM-Z250 | BarX Suzuki |
| Start Position 4 | 67 | Cullin Park | Clermont, FL | Honda CRF250R | Phoenix Racing Honda |
| Start Position 5 | 981 | Austin Politelli | Murrieta, CA | GASGAS MC 250F | Aje Motorsports, Overstock Vehicles, GasGas, Ellison Earthworks, HRT, Burk Mechanical, CCP |
| Start Position 6 | 50 | Marshal Weltin | Ubly, MI | Suzuki RM-Z250 | Progressive Insurance ECSTAR Suzuki |
| Start Position 7 | 41 | Derek Kelley | Riverside, CA | KTM 250 SX-F | AEO Powersports KTM Racing |
| Start Position 8 | 285 | Coty Schock | Dover, DE | Honda CRF250R | Phoenix Racing Honda |
| Start Position 9 | 84 | Mitchell Harrison | Leesburg, FL | GASGAS MC 250F | AJE Motorsport Overstock Vehicles.com GAS GAS |
| Start Position 10 | 243 | Caden Braswell | Shalimar, FL | Honda CRF250R | Phoenix Racing Honda |
| Start Position 11 | 53 | Derek Drake | Lake Elsinore, CA | Suzuki RM-Z250 | BarX Suzuki |
| Start Position 12 | 116 | Tj Albright | Mount Marion, NY | Yamaha YZ250F | 3D Racing Yamaha |
| Start Position 13 | 508 | Hunter Yoder | Menifee, CA | Kawasaki KX250 | Partzilla Prmx Racing, SOBMX, OGS, RynoPower, Ogio |
| Start Position 14 | 330 | A J Catanzaro | Tampa, FL | Honda CRF250R | The Moto Academy, Honda |
| Start Position 15 | 89 | Kaeden Amerine | Great Bend, KS | KTM 250 SX-F | Rocky Mountain ATV MC, Rides Unlimited Racing, Seven MX |
| Start Position 16 | 125 | Luke Neese | Jamestown, NC | Honda CRF250R | Red Research Group, Honda of Lynchburg |
| Start Position 17 | 173 | Hunter Schlosser | El Paso, TX | Yamaha YZ250F | Hugh Ridley, Next Level Racing, FXR, Hole n One, SC Service |
| Start Position 18 | 242 | Garrett Hoffman | Whiteford, MD | Yamaha YZ250F | TPJ, Yamaha, Fly Racing, Oakley, Yoshimura, RTMX, Pirelli |
| Start Position 19 | 85 | Dilan Schwartz | Alpine, CA | Suzuki RM-Z250 | Progressive Insurance Ecstar Suzuki |
| Start Position 20 | 86 | Josiah Natzke | Hamer, SC | Kawasaki KX250 | Partzilla Prmx Racing |
| Start Position 21 | 93 | Jerry Robin | Hamel, MN | GASGAS MC 250F | AJE Motosports |
| Start Position 22 | 289 | Robert Hailey | Las Vegas, NV | Yamaha YZ250F | Jesus, Mom & Dad, Just1, Venom, Brewer, ClubMX |