| POS. | # | RIDER | HOMETOWN | BIKE | TEAM/SPONSORS |
|---|---|---|---|---|---|
| Start Position 1 | 23 | Chase Sexton | Clermont, FL | Honda CRF450R | Team Honda HRC |
| Start Position 2 | 94 | Ken Roczen | Clermont, FL | Suzuki RM-Z450 | Progressive Insurance Ecstar Suzuki |
| Start Position 3 | 1 | Eli Tomac | Cortez, CO | Yamaha YZ450F | Monster Energy Yamaha Star Racing |
| Start Position 4 | 9 | Adam Cianciarulo | New Smyrna Beach, FL | Kawasaki KX450SR | Monster Energy Kawasaki |
| Start Position 5 | 45 | Colt Nichols | Murrieta, CA | Honda CRF450R | Team Honda HRC |
| Start Position 6 | 46 | Justin Hill | Yoncalla, OR | KTM 450 SX-F | Team Tedder Monster Energy Mountain Motorsports |
| Start Position 7 | 12 | Shane McElrath | Oakland, FL | Suzuki RM-Z450 | Twisted Tea Suzuki Progressive Insurance |
| Start Position 8 | 11 | Kyle Chisholm | Valrico, FL | Suzuki RM-Z450 | Twisted Tea Suzuki Progressive Insurance |
| Start Position 9 | 15 | Dean Wilson | Menifee, CA | Honda CRF450R | Fire Power Honda |
| Start Position 10 | 60 | Justin Starling | Riverview, FL | GASGAS MC 450F | FXR, General Grind & Machine, JSR Motorsports |
| Start Position 11 | 751 | Josh Hill | Huntersville, NC | KTM 450 SX-F | Team Tedder Monster Energy Mountain Motorsports |
| Start Position 12 | 78 | Grant Harlan | Decatur, TX | Yamaha YZ450F | Rock River Yamaha |
| Start Position 13 | 80 | Kevin Moranz | Topeka, KS | KTM 450 SX-F | Next Level Racing, Tank Masters, Red Line Oil, KTM |
| Start Position 14 | 111 | Anthony Rodriguez | Tallahasee, FL | Honda CRF450R | Smartop MotoConcepts Racing Honda |
| Start Position 15 | 519 | Joshua Cartwright | Fort Worth, TX | Kawasaki KX450 | Psychic Motorsports, T3 Civil, Ronnie Prado Company, Cardinal |
| Start Position 16 | 460 | Michael Hicks | Fenton, MO | Honda CRF450R WE | TiLube Honda Racing, Storm Lake Honda, FXR, 6D, X Brand |
| Start Position 17 | 68 | Cade Clason | Cameron, NC | Kawasaki KX450 | Partzilla Prmx Racing |
| Start Position 18 | 74 | Logan Karnow | Amherst, OH | Kawasaki KX450 | Only Fans, Oneal, TCD, Rabbit Savior, Kennedy Cycles |
| Start Position 19 | 90 | Tristan Lane | Port Orange, FL | GASGAS MC 450F | Next Level Racing, Silverback Racing, American Recycling Center |
| Start Position 20 | 129 | Lane Shaw | Alvin, TX | GASGAS MC 450F | Ez-Line , Ronnie Prado Company JkopMx, Fly, TJ Cycles |
| Start Position 21 | 170 | Devin Simonson | Laurinburg, NC | Kawasaki KX250 | Partzilla Prmx Racing |
| Start Position 22 | 47 | Fredrik Noren | Indian Trail, NC | Kawasaki KX450SR | MaddParts.com, Big Buildings Direct, Ronnie Prado Company |